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Updated: Jul 31, 2025

Generation of Prostate Cancer Cell Models of Resistance to the Anti-mitotic Agent Docetaxel
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The regulatory effect of cabazitaxel on epithelial-mesenchymal transition in metastatic prostate cancer

Isil Ezgi Eryilmaz1, Gamze Guney Eskiler2, Unal Egeli1

  • 1Department of Medical Biology, Faculty of Medicine, Bursa Uludag University, Bursa, Turkey.

Journal of Cancer Research and Therapeutics
|May 6, 2023
PubMed
Abstract

Insights

Cabazitaxel (Cbx) demonstrates antimetastatic effects in prostate cancer by suppressing epithelial-mesenchymal transition (EMT). This chemotherapy agent downregulates EMT-promoting factors and upregulates specific microRNAs, suggesting a role in controlling cancer metastasis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Epithelial-mesenchymal transition (EMT) is a key driver of cancer metastasis, making its regulation a significant target in anticancer therapies.
  • The precise role of cabazitaxel (Cbx), a chemotherapeutic for metastatic castration-resistant prostate cancer (PC), in regulating EMT within metastatic PC remains incompletely understood.

Purpose of the Study:

  • To investigate the antimetastatic and epithelial-mesenchymal transition (EMT)-regulatory effects of cabazitaxel (Cbx) on hormone-sensitive metastatic prostate cancer (PC) cells.

Main Methods:

  • Anticancer effects of Cbx were assessed using WST-1 and Annexin V assays.
  • Antimetastatic effects were evaluated via wound healing assays and quantitative reverse transcription polymerase chain reaction (qRT-PCR).
  • Analysis focused on EMT-MET markers and EMT-repressor microRNAs (miRNAs) in Cbx-treated LNCaP cells.

Main Results:

  • Cabazitaxel (Cbx) exhibited apoptotic and anti-migratory activities.
  • Cbx demonstrated EMT-repressor effects by downregulating matrix metalloproteinase-9 (MMP-9) and Snail, key EMT promoters.
  • Significant upregulation of miR-205, miR-524, and miR-124 was observed, which are known to repress EMT by targeting EMT-associated gene regulators.

Conclusions:

  • Cabazitaxel (Cbx) possesses EMT-regulatory capabilities beyond its conventional taxane function in hormone-sensitive metastatic prostate cancer (PC).
  • Cbx influences the EMT-MET (mesenchymal-to-epithelial transition) cycling, indicating a potential role in controlling metastatic processes.
Keywords:
Cabazitaxelepithelial-mesenchymal transitionmicroRNAprostate cancersnail

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