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Updated: Jul 31, 2025
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Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
Published on: April 11, 2016
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Application of Comprehensive Genomic Profiling-Based Next-Generation Sequencing Assay to Improve Cancer Care in a
Claudia Cifuentes1, Milton Lombana2, Henry Vargas3
1Clinical Oncology Department, Hospital Universitario Mayor de Mederi, Bogotá, Colombia.
Summary
Comprehensive genomic profiling (CGP) using next-generation sequencing (NGS) identified actionable mutations in over 46% of solid tumors. This approach can guide personalized cancer therapy and improve patient outcomes in developing countries.
Area of Science:
- Oncology
- Genomics
- Molecular Diagnostics
Background:
- Actionable oncogenic mutations are key targets in cancer therapy.
- Comprehensive genomic profiling (CGP) offers a broad approach to identify these mutations.
- Its utility in developing countries requires further investigation.
Purpose of the Study:
- To evaluate the clinical utility of CGP in a developing country.
- To assess the identification of actionable mutations in diverse solid tumors.
- To correlate genomic findings with patient outcomes.
Main Methods:
- Retrospective cohort study of patients with solid tumors (Dec 2016-Nov 2020).
- Hybrid capture-based next-generation sequencing (NGS) for CGP.
- Kaplan-Meier survival analysis for time-to-event variables.
Main Results:
- Actionable mutations were identified in 46.4% of samples.
- Lung primary tumors were the most common (52.9%).
- Median overall survival was 15.5 months; earlier genomic evaluation showed a trend towards improved survival.
Conclusions:
- CGP effectively identifies clinically relevant genomic alterations in various tumors.
- This approach can guide targeted therapy and personalize cancer care in developing nations.
- Genomic profiling aids in achieving better treatment outcomes for cancer patients.
Keywords:
actionable mutationscomprehensive genomic profilingnext-generation sequencingprecision medicineMore Related Videos
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