A selective analysis of sulfamethoxazole - Trimethoprim in tablet formulations using graphene oxide-zinc oxide...

In this study, simultaneous analysis on electrochemical detection of SMX and TMP in tablet formulation has been made using graphene oxide (GO) and ZnO QDs (GO-ZnO QDs) based nanocomposite modified glassy carbon electrode (GCE). The functional group presence was observed using FTIR study. The electrochemical characterization for GO, ZnO QDs and GO-ZnO QDs was studied using cyclic voltammetry using [Fe(CN)₆]^3- medium. In order to estimate the electrochemical redox behavior of SMX and TMP from tablet, the developed electrodes GO/GCE, ZnO QDs/GCE and GO-ZnO QDs/GCE are initially tested for electrochemical activity towards the SMX tablet in BR pH 7 medium. Later their electrochemical sensing has been monitored using square wave voltammetry (SWV). On observing the characteristic behavior of developed electrodes, GO/GCE exhibited detection potential of +0.48 V for SMX and +1.37 V for TMP whereas, ZnO QDs/GCE with +0.78V for SMX and for TMP 1.01 V respectively. Similarly, for GO-ZnO QDs/GCE, its 0.45 V for SMX and 1.11 V for TMP are observed using cyclic voltammetry. The obtained potential results on detecting SMX and TMP are in good agreement with previous results. Under optimized conditions, the response has been monitored with linear concentration range 50 μg/L to 300 μg/L for GO/GCE, ZnO QDs/GCE and GO-ZnO QDs/GCE in SMX tablet formulations. Their detection limits for the individual detection using GO-ZnO/GCE for SMX and TMP are found to be 0.252 ng/L and 19.10 μg/L and for GO/GCE it was 0.252 pg/L and 2.059 ng/L respectively. It was observed that ZnO QDs/GCE could not provide the electrochemical sensing towards SMX and TMP which may be due to the ZnO QPs can act as a blocking layer impeding the electron transfer process. Thus, the sensor performance lead to promising biomedical applications in real-time monitoring on evaluating selective analysis with SMX and TMP in tablet formulations.