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Updated: Jul 31, 2025

Measuring Plant Cell Wall Extension Creep Induced by Acidic pH and by Alpha-Expansin
Published on: March 11, 2009
Biomechanical Weakening of Paper and Plant Cell Walls by Bacterial Expansins
Daniel J Cosgrove1, Nathan K Hepler2, Edward R Wagner2
1Department of Biology, Pennsylvania State University, University Park, PA, USA. dcosgrove@psu.edu.
Abstract:
Expansins are proteins that loosen plant cell walls but lack enzymatic activity. Here we describe two protocols tailored to measure the biomechanical activity of bacterial expansin. The first assay relies on the weakening of filter paper by expansin. The second assay is based on induction of creep (long-term, irreversible extension) of plant cell wall samples.
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