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Deciphering and advancing CAR T-cell therapy with single-cell sequencing technologies
Shengkang Huang1,2, Xinyu Wang1, Yu Wang3
1The Second School of Clinical Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, China.
Abstract:
Chimeric antigen receptor (CAR) T-cell therapy has made remarkable progress in cancer immunotherapy, but several challenges with unclear mechanisms hinder its wide clinical application. Single-cell sequencing technologies, with the powerful unbiased analysis of cellular heterogeneity and molecular patterns at unprecedented resolution, have greatly advanced our understanding of immunology and oncology. In this review, we summarize the recent applications of single-cell sequencing technologies in CAR T-cell therapy, including the biological characteristics, the latest mechanisms of clinical response and adverse events, promising strategies that contribute to the development of CAR T-cell therapy and CAR target selection. Generally, we propose a multi-omics research mode to guide potential future research on CAR T-cell therapy.
Insights
Single-cell sequencing advances chimeric antigen receptor (CAR) T-cell therapy by revealing cellular details. This review explores its use in understanding CAR T-cell responses, adverse events, and improving therapy development.
Area of Science:
- Immunology
- Oncology
- Biotechnology
Background:
- Chimeric antigen receptor (CAR) T-cell therapy shows promise in cancer immunotherapy.
- Challenges with unclear mechanisms impede the widespread clinical use of CAR T-cell therapy.
Purpose of the Study:
- To review the applications of single-cell sequencing technologies in CAR T-cell therapy.
- To explore how single-cell sequencing enhances understanding of CAR T-cell biology, clinical responses, and adverse events.
- To discuss strategies for CAR T-cell therapy development and target selection.
Main Methods:
- Review of recent literature on single-cell sequencing applications in CAR T-cell therapy.
- Analysis of biological characteristics, clinical response mechanisms, and adverse events.
- Examination of strategies for CAR T-cell therapy development and CAR target selection.
Main Results:
- Single-cell sequencing provides high-resolution analysis of cellular heterogeneity and molecular patterns.
- These technologies have significantly advanced the understanding of immunology and oncology in the context of CAR T-cell therapy.
- Applications include characterizing CAR T-cells, elucidating response/adverse event mechanisms, and guiding therapy development.
Conclusions:
- Single-cell sequencing is crucial for dissecting CAR T-cell therapy complexities.
- A multi-omics approach is proposed to guide future research in this field.
- Further research using these technologies will optimize CAR T-cell therapy efficacy and safety.
