Related Experiment Video
Updated: Jul 31, 2025

Confocal Imaging of Confined Quiescent and Flowing Colloid-polymer Mixtures
Published on: May 20, 2014
Fickian yet non-Gaussian diffusion of a quasi-2D colloidal system in an optical speckle field: experiment and
Antonio Ciarlo1, Raffaele Pastore2, Francesco Greco2
1Department of Physics E. Pancini, University of Naples Federico II, Complesso Universitario Monte Sant'Angelo, Via Cintia, 80126, Naples, Italy. antonio.ciarlo@unina.it.
Abstract:
We investigate a quasi-2D suspension of Brownian particles in an optical speckle field produced by holographic manipulation of a laser wavefront. This system was developed to study, in a systematic and controllable way, a distinctive instance of diffusion, called Fickian yet Non Gaussian diffusion (FnGD), observed, during the last decade, for colloidal particles in a variety of complex and biological fluids. Our setup generates an optical speckle field that behaves like a disordered set of optical traps. First, we describe the experimental setup and the dynamics of the particles, focusing on mean square displacements, displacement distributions and kurtosis. Then, we present Brownian Dynamics simulations of point-like particles in a complex energy landscape, mimicking that generated by the optical speckle field. We show that our simulations can capture the salient features of the experimental results, including the emergence of FnGD, also covering times longer than the ones so far achieved in experiments. Some deviations are observed at long time only, with the Gaussian restoring being slower in simulations than in experiments. Overall, the introduced numerical model might be exploited to guide the design of upcoming experiments targeted, for example, to fully monitor the recovery of Gaussianity.
More Related Videos
05:56Spot Variation Fluorescence Correlation Spectroscopy for Analysis of Molecular Diffusion at the Plasma Membrane of Living Cells
Published on: November 12, 2020
15:10From Fast Fluorescence Imaging to Molecular Diffusion Law on Live Cell Membranes in a Commercial Microscope
Published on: October 9, 2014