Protocol for electrotaxis of large epithelial cell sheets
Related Experiment Video
Updated: Jul 31, 2025
Electric Field-controlled Directed Migration of Neural Progenitor Cells in 2D and 3D Environments
Published on: February 16, 2012
Yan Zhang1, Rachel M Lee2, Zijie Zhu3
1Department of Ophthalmology & Vision Science, University of California, California, Davis, CA 95616, USA; School of Public Health, Hangzhou Normal University, Hangzhou 311121, China; Institute of Environmental Medicine, Zhejiang University School of Medicine, Hangzhou 310058, China; Micro-Nano Innovations (MiNI) Laboratory, Department of Biomedical Engineering, University of California, California, Davis, CA 95616, USA.
Abstract:
Here, we present a protocol for electrotaxis of large epithelial cell sheets without compromising the integrity of cell epithelia in a high-throughput customized directed current electrotaxis chamber. We describe the fabrication and use of polydimethylsiloxane stencils to control the size and shape of human keratinocyte cell sheets. We detail cell tracking, cell sheet contour assay, and particle image velocimetry to reveal the spatial and temporal motility dynamics of cell sheets. This approach is applicable to other collective cell migration studies. For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).1.
