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Updated: Jul 31, 2025
Development of a Direct Pulp-capping Model for the Evaluation of Pulpal Wound Healing and Reparative Dentin Formation in Mice
Published on: January 12, 2017
Human Digested Dentin Matrix for Dentin Regeneration and the Applicative Potential in Vital Pulp Therapy
Zheng Li1, Chengxiang Zheng1, Peiru Jiang1
16 Stomatological Hospital of Chongqing Medical University, Chongqing, China; Chongqing Key Laboratory of Oral Diseases and Biomedical Sciences, Chongqing Medical University, Chongqing, China; Chongqing Municipal Key Laboratory of Oral Biomedical Engineering of Higher Education, Chongqing Medical University, Chongqing, China.
This study developed a novel dentin matrix material from human dentin for vital pulp therapy. The biomaterial promotes dentin regeneration and shows potential for pulp capping applications.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Dental Research
Background:
- Human dentin, a natural acellular matrix, exhibits excellent biocompatibility.
- Vital pulp therapy aims to preserve pulp vitality, requiring effective biomaterials.
- Current materials for pulp capping have limitations in promoting natural tissue regeneration.
Purpose of the Study:
- To fabricate a novel dentin matrix material from human dentin.
- To investigate the biocompatibility and odontogenic potential of the new material.
- To evaluate its efficacy in vital pulp therapy.
Main Methods:
- Fabrication of digested dentin matrix extract (DDME) via enzymatic digestion.
- Assessment of DDME surface properties and biocompatibility in vitro.
- Evaluation of DDME's effect on human dental pulp cells (hDPCs) differentiation and mineralization in vivo.
- Testing DDME as a pulp capping agent in a rat pulpotomy model.
- Analysis of molecular mechanisms using mRNA sequencing and Hippo signaling pathway investigation.
Main Results:
- A novel dentin matrix material (DDME) with uniform particle size was successfully fabricated.
- DDME demonstrated biocompatibility, enhancing odontogenic differentiation markers (e.g., DMP-1, COL1A1) in hDPCs without affecting viability.
- DDME outperformed HA-TCP in inducing dentin-like mineralization in vivo and showed curative effects in a rat pulpotomy model.
- The mechanism involves Hippo signaling inhibition and YAP1 nuclear translocation, promoting DMP-1 expression.
Conclusions:
- Human digested dentin matrix extract (DDME) is a promising biomaterial for dentin regeneration.
- DDME shows significant potential as a component of vital pulp therapy, possibly in combination with existing agents.
