Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

GPCR Desensitization01:12

GPCR Desensitization

6.2K
G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
6.2K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

2.1K
Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.1K
Transducer Mechanism: Enzyme-Linked Receptors01:27

Transducer Mechanism: Enzyme-Linked Receptors

2.6K
Enzyme-linked receptors are cell-surface receptors acting as an enzyme or associating with an enzyme intracellularly. They make excellent drug targets. Drugs can bind to the extracellular ligand-binding domain or directly affect their enzymatic domain and alter their activity.
Major types that are helpful drug targets include:
2.6K
Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

8.5K
When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
8.5K
IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

12.2K
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
12.2K
Directing Proteins to the Rough Endoplasmic Reticulum01:34

Directing Proteins to the Rough Endoplasmic Reticulum

7.3K
The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
7.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The CLE9/CLE10 signaling peptides control de novo shoot regeneration in Arabidopsis.

Plant physiology and biochemistry : PPB·2025
Same author

Enhancement growth, water use efficiency and economic benefit for maize by drip irrigation in Northwest China.

Scientific reports·2023
Same author

DNA controllable peroxidase-like activity of Ti<sub>3</sub>C<sub>2</sub> nanosheets for colorimetric detection of microcystin-LR.

Analytical and bioanalytical chemistry·2023
Same author

Identification and functional characterization of AcMYB113 in anthocyanin metabolism of Aesculus chinensis Bunge var. chinensis leaves.

Plant physiology and biochemistry : PPB·2023
Same author

Effect of dual residual risk of cholesterol and inflammation on all-cause mortality in patients with cardiovascular disease.

Cardiovascular diabetology·2023
Same author

The correlation of sacral table angle to spinopelvic sagittal alignment in healthy adults.

Journal of orthopaedic surgery and research·2023
Same journal

Better breeding leveraging more biology.

Trends in plant science·2026
Same journal

Women in plant science around the world.

Trends in plant science·2026
Same journal

Bilateral symmetry genes: If they exist, how would we know?

Trends in plant science·2026
Same journal

From xylem atlases to developmental continuity in forestry.

Trends in plant science·2026
Same journal

Small peptides guard the gate of plant immunity.

Trends in plant science·2026
Same journal

Phosphorylation blues: Cracking the phototropin phosphocode.

Trends in plant science·2026
See all related articles

Related Experiment Video

Updated: Jul 31, 2025

Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells
12:38

Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells

Published on: November 6, 2021

2.6K

pH regulates peptide-receptor perception.

Huibin Han1, Alina Glazunova2, Guodong Wang2

  • 1College of Bioscience and Bioengineering, Jiangxi Agricultural University, Jiangxi, Nanchang, 330045, China.

Trends in Plant Science
|May 7, 2023
PubMed
Summary
This summary is machine-generated.

Plant small peptides signal through receptors for communication, but how this occurs is unclear. New research reveals that pH is a key regulator in plant peptide-receptor perception and signaling.

Keywords:
pHpeptide–receptor interactionplant developmentsmall peptide

More Related Videos

Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking
09:59

Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking

Published on: March 16, 2017

8.9K
Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
19:05

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Published on: October 30, 2015

12.4K

Related Experiment Videos

Last Updated: Jul 31, 2025

Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells
12:38

Screening Peptides that Activate MRGPRX2 using Engineered HEK Cells

Published on: November 6, 2021

2.6K
Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking
09:59

Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking

Published on: March 16, 2017

8.9K
Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay
19:05

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

Published on: October 30, 2015

12.4K

Area of Science:

  • Plant biology
  • Molecular signaling
  • Cell communication

Background:

  • Plant small peptides are crucial signaling molecules.
  • Intercellular communication via peptides regulates plant development and adaptation.
  • The precise mechanisms of peptide perception by receptors are not fully understood.

Purpose of the Study:

  • To elucidate the mechanisms underlying peptide-receptor perception in plants.
  • To investigate the role of environmental factors in modulating peptide signaling.
  • To provide insights into how pH affects plant peptide perception.

Main Methods:

  • Analysis of peptide-receptor interactions under varying pH conditions.
  • Biochemical assays to study binding affinities.
  • Cellular imaging to observe signaling events.

Main Results:

  • pH significantly influences the perception and binding of plant small peptides to their receptors.
  • Specific pH ranges optimize or inhibit peptide-receptor complex formation.
  • This pH-dependent regulation impacts downstream signaling pathways.

Conclusions:

  • pH is a critical environmental cue that modulates plant peptide-receptor perception.
  • Understanding pH-mediated signaling offers new avenues for controlling plant growth and responses.
  • These findings advance our knowledge of intercellular communication in plants.