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Updated: Jul 31, 2025

Non-invasive Optical Imaging of the Lymphatic Vasculature of a Mouse
Published on: March 8, 2013
Visualizing the lymphatic vessels and flow with high-resolution ultrasonography and microvascular flow imaging
Joep J J Vullings1, Caroline van Schaik1, Jurgen J Fütterer1
1Department of Medical Imaging Radiology, Radboud University Medical Center, Nijmegen, The Netherlands.
Abstract:
Imaging of the lymphatic system has always encountered difficulties, such as high costs, timeconsuming procedures, and low-resolution images. Each method of imaging provides its own challenges. The use of high-resolution ultrasonography (HR-US) and microvascular flow imaging (MVFI) may prove to be the most effective method for visualizing the superficial lymphatic vessels. This study investigated the utilization of HR-US and MVFI in imaging inguinal lymph nodes and vessels, as well as the innovative use of an intranodal saline injection that acted as a contrast agent. This technical note aims to demonstrate that HR-US and MVFI, in combination with an intranodal saline injection, are applicable to the dynamic imaging of superficial inguinal lymph nodes and vessels.

