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Immunosensor for Rapid and Sensitive Detection of Digoxin.

Shuyang Zhao1, Ruxue Zhang1, Yujie Gao1

  • 1School of Life Science and Technology, Weifang Medical University, Weifang 261053, China.

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This study developed a rapid immunofluorescent sensor for detecting digoxin, a heart medication that can be toxic. The new method allows for quick and accurate measurement of blood digoxin levels, aiding therapeutic drug monitoring and preventing poisoning.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Pharmacology

Background:

  • Digoxin is a vital cardiac drug for heart conditions but has a narrow therapeutic index, posing a risk of toxicity.
  • Accurate and timely monitoring of digoxin blood concentration is crucial for patient safety and effective treatment.
  • Existing methods for digoxin detection may lack the speed and sensitivity required for rapid therapeutic drug monitoring.

Purpose of the Study:

  • To construct a DNA vector for expressing the antidigoxin antibody Fab fragment.
  • To develop a novel immunofluorescent sensor for rapid digoxin detection.
  • To evaluate the performance of the sensor for therapeutic drug monitoring.

Main Methods:

  • Construction of a DNA vector for antidigoxin antibody Fab fragment expression in E. coli.
  • Purification of the expressed Fab fragment and labeling with fluorescent dyes.
  • Development of a photoinduced electron transfer-based immunofluorescent sensor.

Main Results:

  • Successfully expressed and purified 1.2 mg of antidigoxin antibody Fab fragment from 100 mL culture.
  • Developed an immunofluorescent sensor with a rapid assay time of under 5 minutes.
  • Achieved a limit of detection of 0.023 ng/mL and a wide detectable range (0.023 ng/mL to 100 μg/mL).

Conclusions:

  • A novel, rapid immunofluorescent sensor for digoxin detection has been successfully developed.
  • The sensor demonstrates high sensitivity and a broad detection range, suitable for therapeutic drug monitoring.
  • This approach offers a significant advancement for timely digoxin level assessment, improving patient outcomes and reducing toxicity risks.