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Updated: Jul 31, 2025
09:17
Co-immunoprecipitation Assay Using Endogenous Nuclear Proteins from Cells Cultured Under Hypoxic Conditions
Published on: August 2, 2018
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The RNA-binding protein Snd1/Tudor-SN regulates hypoxia-responsive gene expression
Juha Saarikettu1, Saara Lehmusvaara2, Marko Pesu2,3
1Institute of Biotechnology, HiLIFE Helsinki Institute of Life Sciences University of Helsinki Helsinki Finland.
FASEB Bioadvances
|May 8, 2023
Summary
The study reveals that the Snd1 protein regulates gene expression and plays a role in the body's adaptation to low oxygen conditions (hypoxia). Snd1 negatively controls hypoxia-induced microRNAs and transcription.
Area of Science:
- Molecular Biology
- Gene Regulation
- Cellular Stress Response
Background:
- Snd1 is an evolutionarily conserved RNA-binding protein involved in gene expression regulation.
- Its precise function in mammalian physiology, particularly under stress, requires further elucidation.
Purpose of the Study:
- To investigate the physiological consequences of Snd1 gene deletion in mice.
- To understand Snd1's role in cellular responses, specifically adaptation to hypoxia.
- To identify proteins interacting with Snd1.
Main Methods:
- Generation and analysis of Snd1 knockout mice.
- Transcriptomic analysis (mRNA and microRNA) in spleen and liver.
- BioID proximity ligation assay in HEK-293T cells.
Main Results:
- Snd1 knockout mice exhibited reduced fertility, body size, and myeloid cell counts.
- Gene expression changes in knockout livers mirrored hypoxia adaptation profiles.
- Upregulation of hypoxia-induced microRNAs (miR-96, -182) was observed in knockout livers.
- SND1 interactors identified via BioID were predominantly RNA-binding proteins.
Conclusions:
- Snd1 is not essential for mRNA transcription under normal conditions but is crucial for hypoxia adaptation.
- Snd1 negatively regulates hypoxia-responsive microRNAs and transcription, acting as a stress response regulator.
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