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Updated: Jul 31, 2025

Author Spotlight: A Novel Cell Injection Method with Minimum Invasion
06:24

Author Spotlight: A Novel Cell Injection Method with Minimum Invasion

Published on: April 21, 2023

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A Novel Cell Injection Method with Minimum Invasion.

Fumiyuki Hattori1

  • 1Innovative Regenerative Medicine, Graduate School of Medicine, Kansai Medical University; hattorif@hirakata.kmu.ac.jp.

Journal of Visualized Experiments : Jove
|May 8, 2023
PubMed
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A novel slow injection method using temperature-sensitive gelatin solution enables effective cell delivery for cardiac repair. This technique minimizes tissue injury and promotes cardiomyocyte engraftment, improving heart function after myocardial infarction.

Area of Science:

  • Regenerative Medicine
  • Biomaterials Science
  • Cardiovascular Research

Background:

  • Cell injection for tissue repair can cause invasive injury due to suspension solution volume.
  • High injection speeds are typically needed, creating a conflict with minimizing tissue damage.

Purpose of the Study:

  • To develop a novel, minimally invasive cell injection method to improve cell transplantation outcomes.
  • To investigate the efficacy of a slow injection technique using a non-Newtonian fluid for cardiac cell delivery.

Main Methods:

  • A slow injection technique was employed using a temperature-sensitive gelatin solution as the cell suspension.
  • The gelatin solution was maintained in a gel state by cooling the syringe and transitioned to a sol state at body temperature post-injection.

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Last Updated: Jul 31, 2025

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  • Purified neonatal rat cardiomyocytes were injected into myocardial infarction sites in adult rat hearts.

    • Main Results:

    • The slow injection method successfully delivered cardiomyocyte aggregates without causing surrounding fibrosis.
    • Transplanted hearts showed significantly improved contractile function at 2 months post-injection.
    • Histological analysis revealed seamless integration of host and graft cardiomyocytes, including functional gap junctions.

    Conclusions:

    • The slow injection technique using temperature-sensitive gelatin is a promising approach for cardiac regenerative medicine.
    • This method reduces injection-related tissue injury and enhances the functional integration of transplanted cardiomyocytes.
    • The findings support the potential of this technique for next-generation cell therapies in treating heart disease.