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Updated: Jul 31, 2025

Measurement of BK-polyomavirus Non-Coding Control Region Driven Transcriptional Activity Via Flow Cytometry

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Published on: July 13, 2019

Single-Cell, High-Content Microscopy Analysis of BK Polyomavirus Infection.

Megan C Procario¹, Jonathan Z Sexton^2,3,4, Benjamin S Halligan²

¹Department of Microbiology and Immunology, Medical School, University of Michigan, Ann Arbor, Michigan, USA.

Microbiology Spectrum

|May 8, 2023

Summary

This summary is machine-generated.

BK polyomavirus (BKPyV) infects most people, but disease only occurs in the immunocompromised. This study reveals significant differences in BKPyV infection dynamics at the single-cell level, challenging previous assumptions from bulk population studies.

Keywords:

BKPyV high-content microscopy polyomavirus single-cell infection

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Area of Science:

Virology
Cell Biology
Immunology

Background:

BK polyomavirus (BKPyV) establishes persistent infections in the majority of the adult population.
BKPyV disease primarily affects transplant recipients on immunosuppressants, with limited treatment options.
Previous studies relied on bulk cell populations, assuming uniform infection dynamics.

Purpose of the Study:

To investigate BKPyV infection dynamics at the single-cell level.
To analyze viral protein (TAg), PML, DNA, and nuclear morphology in individual infected cells.
To establish a novel high-content, single-cell microscopy assay for BKPyV research.

Main Methods:

High-content microscopy was employed to analyze BKPyV infection in single cells.
Measurements included viral protein large T antigen (TAg), PML, DNA, and nuclear morphology.
Data were analyzed to identify heterogeneity in infection parameters across individual cells and time points.

Main Results:

Significant heterogeneity was observed in BKPyV infection among individual cells.
TAg levels within cells did not consistently increase over time.
Cells with similar TAg levels exhibited distinct characteristics, indicating complex infection dynamics.

Conclusions:

High-content, single-cell microscopy provides novel insights into the heterogeneous nature of BKPyV infection.
This approach challenges assumptions derived from bulk population studies.
The developed assay is a powerful tool for future BKPyV research and understanding viral biology.