Biochemical and SSR based molecular characterization of elite rice varieties for straw lignocellulose
Abinash Mishra1, Jyoti Prakash Sahoo1, Bishnupriya Swain2
1Department of Plant Breeding and Genetics, College of Agriculture, Odisha University of Agriculture & Technology, Bhubaneswar, Odisha, India.
Background:
Lignocellulosic biomass from rice straw possesses enormous potential in generating bioenergy thereby reducing the dependence of human on non-renewable fuel sources. Developing rice varieties of such calibre necessitates biochemical characterization as well as assessing the presence of genetic diversity among the rice genotypes with respect to cellulose content.
Methods And Results:
Forty-three elite rice genotypes were selected for biochemical characterization and SSR marker-based genetic fingerprinting. For genotyping, 13 cellulose synthase specific polymorphic markers were used. The diversity analysis was performed using TASSEL 5.0 and GenAlE × 6.51b2, software program. Of the 43 rice varieties, CR-Dhan-601, CR-Dhan-1014, Mahanadi, Jagabandhu, Gouri, Samanta and Chandrama were found to possess desirable lignocellulosic composition with respect to harnessing green fuels. The marker OsCESA-1.3 expressed the highest PIC (0.640), while the marker OsCESA-6.3 of lowest PIC (0.128). A moderate average estimate (0.367) of PIC was observed under current set of genotypes and marker system. The dendrogram analysis grouped the rice genotypes into two principal clusters i.e., cluster I and II. Cluster-II is monogenetic, while cluster-I is having 42 genotypes.
Conclusions:
The moderate level of both PIC and H average estimates indicate the narrow genetic bases of the germplasms. The varieties falling under different clusters possessing desirable lignocellulosic composition can be used in a hybridization programme to develop bioenergy efficient varieties. The promising varietal combinations that can be used as parents for developing bioenergy efficient genotypes are Kanchan / Gobinda, Mahanadi / Ramachandi, Mahanadi / Rambha, Mahanadi / Manika, Rambha / Manika, Rambha / Indravati and CR-Dhan-601 / Manika as they offer an advantage of higher cellulose accumulation. This study helped in identification of suitable dual purpose rice varieties for biofuel production without compromising food security.
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