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Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization
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Fourier holographic endoscopy for imaging continuously moving objects.

Munkyu Kang, Wonjun Choi, Wonshik Choi

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    This study introduces a novel holographic method for fiber bundle endoscopy, enabling high-resolution, pixelation-free imaging of moving objects. This overcomes limitations of static imaging, allowing real-time monitoring in dynamic environments.

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    Area of Science:

    • Optics and Photonics
    • Biomedical Imaging
    • Endoscopy

    Background:

    • Conventional fiber bundle endoscopy relies on distal optics, leading to pixelated images.
    • Holographic reflection matrix recording offers pixelation-free imaging but requires static probes.
    • Existing methods struggle with imaging moving objects due to probe motion altering phase retardations.

    Purpose of the Study:

    • To develop a method for high-resolution endoscopic imaging through a fiber bundle capable of adapting to probe movement.
    • To enable real-time imaging of dynamic scenes using a fiber optic endoscope.
    • To overcome the limitations of static reflection matrix recording for moving objects.

    Main Methods:

    • Acquired reflection matrices from a Fourier holographic endoscope with a fiber bundle.
    • Investigated the impact of fiber bending on the recorded reflection matrix.
    • Developed a motion compensation technique to correct for fiber bundle perturbations.

    Main Results:

    • Demonstrated high-resolution endoscopic imaging through a dynamic fiber bundle.
    • Successfully resolved reflection matrix perturbations caused by a moving fiber probe.
    • Achieved stable imaging despite continuous changes in the fiber probe's shape.

    Conclusions:

    • The developed method enables robust, high-resolution endoscopic imaging through flexible fiber bundles, even with continuous motion.
    • This technique is suitable for minimally invasive monitoring of dynamic biological processes, such as behaving animals.
    • Pixelation-free imaging of moving objects is now feasible with adaptable fiber optic probes.