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Probiotic-fermented Portulaca oleracea L. alleviated DNFB-induced atopic dermatitis by inhibiting the NF-κB signaling
Wenjun Zhao1, Yuwei Zhang1, Weijie Li1
1College of Food Science, South China Agricultural University, Guangzhou, 510642, China.
Ethnopharmacological Relevance:
Probiotic fermentation is a mild and safe biological method to boost the performance of herbs. Portulaca oleracea L. (PO), with folklore records of purgative, anti-dermatological and anti-epidemic effects, has been demonstrated to possess anti-inflammatory, immunomodulatory, and antioxidant properties. However, the potential of PO for the treatment of atopic dermatitis (AD) has not been sufficiently explored.
Aim Of Study:
This study aimed to evaluate the therapeutic benefits of PO and fermented Portulaca oleracea L. (FPO) and explore their intrinsic mechanisms.
Methods:
By utilizing 2,4-dinitrofluorobenzene-induced AD mice as a model, the histopathology of the lesions was observed using H&E and toluidine blue staining methods; the levels of immunoglobulin E (Ig E), histamine (HIS), and thymic stromal lymphopoietin (TSLP) in serum were measured using ELISA, whereas, the expression of inflammatory cytokines in skin lesion was measured using ELISA and immunohistochemistry experiments. The expression of tumor necrosis factor-α (TNF-α), IKKα, NF-κB mRNA was measured using qPCR; and the expression of TNF-α、p-IKKα, p-IκBα, p-NF-κB was measured using western blotting.
Results:
Both 20 mg/mL PO and FPO alleviated mast cell infiltration and lesion pathology, reduced serum levels of Ig E, HIS and TSLP, down-regulated the expression of AD-related inflammatory cytokines, such as, TNF-α, interferon-γ, and interleukin-4, and increased filaggrin expression. Furthermore, they inhibited the expression of TNF-α, IKKα, and NF-κB genes and TNF-α, p-IKKα, p-NF-κB and p-IκBα proteins associated with the NF-κB signaling pathway.
Conclusions:
PO and FPO has a positive therapeutic potential on AD, indicating that it may be employed as alternative therapies for AD.
Insights
Portulaca oleracea L. (PO) and its fermented form (FPO) show therapeutic potential for atopic dermatitis (AD). These treatments reduced inflammation and improved skin lesions by modulating key immune pathways.
Area of Science:
- Herbal Medicine
- Immunology
- Dermatology
Background:
- Probiotic fermentation enhances herbal properties.
- Portulaca oleracea L. (PO) has known anti-inflammatory and antioxidant effects.
- The therapeutic potential of PO for atopic dermatitis (AD) remains underexplored.
Purpose of the Study:
- To evaluate the therapeutic benefits of PO and fermented Portulaca oleracea L. (FPO) on atopic dermatitis.
- To investigate the underlying mechanisms of action for PO and FPO in AD treatment.
Main Methods:
- A mouse model of 2,4-dinitrofluorobenzene-induced atopic dermatitis was used.
- Histopathology, serum marker analysis (Ig E, histamine, TSLP), cytokine expression (ELISA, immunohistochemistry), gene expression (qPCR), and protein expression (western blotting) were employed.
- Key pathways, including NF-κB signaling, were assessed.
Main Results:
- Both PO and FPO significantly alleviated mast cell infiltration and lesion pathology in AD mice.
- Treatment reduced serum levels of Ig E, histamine, and TSLP, and down-regulated pro-inflammatory cytokines.
- PO and FPO inhibited the NF-κB signaling pathway and increased filaggrin expression.
Conclusions:
- Portulaca oleracea L. (PO) and its fermented form (FPO) demonstrate significant therapeutic potential for atopic dermatitis.
- These findings suggest that PO and FPO could serve as alternative therapies for managing AD.
- The study elucidates the anti-inflammatory mechanisms involving the NF-κB pathway.
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