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Legionella pneumophila-mediated host posttranslational modifications

Yi Yang1, Ligang Mei1, Jing Chen1

  • 1School of Life Sciences, Chongqing University, Chongqing 401331, China.

Journal of Molecular Cell Biology
|May 8, 2023
PubMed

Insights

Legionella pneumophila manipulates host cells using effector proteins that add or remove posttranslational modifications (PTMs). This review details how these PTMs impact bacterial survival and host immune evasion.

Area of Science:

  • Microbiology
  • Cell Biology
  • Biochemistry

Background:

  • Legionella pneumophila, a Gram-negative bacterium, causes Legionnaires' disease pneumonia.
  • It utilizes an Icm/Dot type IV secretion system to deliver over 300 effector proteins into host cells.
  • These effectors manipulate host defenses for bacterial survival and replication.

Approach:

  • This review summarizes effector-mediated posttranslational modifications (PTMs) of host proteins by L. pneumophila.
  • It covers both the addition and removal of various PTMs, including phosphorylation, ubiquitination, glycosylation, AMPylation, phosphocholination, methylation, and ADP-ribosylation.
  • The molecular mechanisms and biological functions of these PTMs are described.

Key Points:

  • L. pneumophila effectors mediate diverse PTMs on host proteins, including phosphorylation, ubiquitination, glycosylation, AMPylation, phosphocholination, methylation, and ADP-ribosylation.
  • Effectors also remove PTMs through dephosphorylation, deubiquitination, deAMPylation, deADP-ribosylation, dephosphocholination, and delipidation.
  • These modifications are crucial for regulating bacterial growth, Legionella-containing vacuole biogenesis, and host immune disruption.

Conclusions:

  • L. pneumophila extensively employs effector-mediated PTMs to subvert host cell processes.
  • Understanding these PTMs provides insights into bacterial pathogenesis and host-pathogen interactions.
  • Targeting these effector functions could offer novel therapeutic strategies against Legionnaires' disease.
Keywords:
Legionella pneumophilaLegionella-containing vacuolebacterial effectorhost defense systemhost–pathogen interactionpathogenesisposttranslational modification

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