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High-contrast en bloc staining of mouse whole-brain and human brain samples for EM-based connectomics
Kun Song1, Zhihui Feng2, Moritz Helmstaedter3
1Department of Connectomics, Max Planck Institute for Brain Research, Frankfurt, Germany. kun.song@brain.mpg.de.
Nature Methods
|May 8, 2023
Abstract:
Connectomes of human cortical gray matter require high-contrast homogeneously stained samples sized at least 2 mm on a side, and a mouse whole-brain connectome requires samples sized at least 5-10 mm on a side. Here we report en bloc staining and embedding protocols for these and other applications, removing a key obstacle for connectomic analyses at the mammalian whole-brain level.
