Related Experiment Video

Updated: Jul 31, 2025

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
04:25

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

Published on: October 28, 2021

9.5K

Genipin Inhibits the Development of Osteosarcoma through PI3K/AKT Signaling Pathway

Xiongjie Huang1, Habu Jiwa1, Jingtao Xu1

  • 1Department of Orthopedics, The First Affiliated Hospital of Chongqing Medical University, Chongqing, 400016, China.

Current Pharmaceutical Design
|May 9, 2023
PubMed
Abstract

Insights

Genipin, a natural compound, effectively inhibits osteosarcoma cell growth, migration, and invasion while promoting apoptosis. This study suggests genipin

Area of Science:

  • Oncology
  • Pharmacology
  • Natural Products

Background:

  • Osteosarcoma is an aggressive bone cancer with limited treatment options.
  • Current chemotherapy for osteosarcoma causes significant side effects.
  • Genipin, derived from Gardenia, exhibits diverse pharmacological properties.

Purpose of the Study:

  • To evaluate the anti-cancer effects of genipin on osteosarcoma.
  • To elucidate the underlying molecular mechanisms of genipin's action.

Main Methods:

  • Cell proliferation assays (crystal violet, MTT, colony formation).
  • Cell migration and invasion assays (scratch healing, Transwell).
  • Apoptosis assays (Hoechst staining, flow cytometry) and Western blot for protein expression.
  • In vivo studies using an orthotopic osteosarcoma model.

Main Results:

  • Genipin significantly suppressed osteosarcoma cell proliferation, migration, and invasion.
  • Genipin induced apoptosis in osteosarcoma cells.
  • Genipin demonstrated anti-tumor efficacy in vivo, potentially via the PI3K/AKT pathway.

Conclusions:

  • Genipin exhibits potent anti-osteosarcoma activity.
  • Genipin's mechanism involves inhibiting osteosarcoma cell growth and inducing apoptosis.
  • The PI3K/AKT signaling pathway is implicated in genipin's anti-cancer effects.
Keywords:
GenipinPI3K/AKT signalingapoptosismigrationosteosarcomaproliferation

More Related Videos

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate07:31

A Syngeneic Orthotopic Osteosarcoma Sprague Dawley Rat Model with Amputation to Control Metastasis Rate

Published on: May 3, 2021

3.7K
Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment11:13

Author Spotlight: Exploring Salidroside's Molecular Mechanisms in Breast Cancer Treatment

Published on: June 9, 2023

1.6K

Related Concept Videos

PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
3.7K
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
9.0K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K
Inhibition of Cdk Activity02:34

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...
4.8K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.3K