Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

13.4K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
13.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The subpleural pulmonary microvasculature in newborn yak (Bos grunniens).

Veterinary research communications·2008
Same author

Experimental confirmation of potential swept source optical coherence tomography performance limitations.

Applied optics·2008
Same author

A germin-like protein gene family functions as a complex quantitative trait locus conferring broad-spectrum disease resistance in rice.

Plant physiology·2008
Same author

[Spatial and temporal changes of palatal cell proliferation and cell apoptosis of retinoic acid induced mouse cleft palate in different embryonic stages].

Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology·2008
Same author

Identification of an Atlantic salmon IFN multigene cluster encoding three IFN subtypes with very different expression properties.

Developmental and comparative immunology·2008
Same author

Non-Gaussian statistics and superdiffusion in a driven-dissipative dusty plasma.

Physical review. E, Statistical, nonlinear, and soft matter physics·2008

Related Experiment Video

Updated: Jul 31, 2025

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
10:53

Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

Published on: January 3, 2017

9.9K

Non-defocus high transmittance image slicer.

Ruyi Wei, Bin Liu, Zhengmao Xie

    Optics Express
    |May 9, 2023
    PubMed
    Summary

    A novel optical path compensation method using a stepped prism plate eliminates defocus in high-transmittance image slicers. This breakthrough significantly reduces image blur, enhancing performance for spectrometers.

    Area of Science:

    • Optics
    • Spectroscopy
    • Optical Engineering

    Background:

    • Traditional image slicers often suffer from image blur due to defocus between sub-images.
    • This defocus issue limits the resolution and performance of spectroscopic instruments.

    Purpose of the Study:

    • To develop the first non-defocus, high-transmittance image slicer.
    • To address and mitigate image blur in sliced sub-images using optical path compensation.

    Main Methods:

    • Proposed an optical path compensation method utilizing a stepped prism plate.
    • Designed and simulated the performance of the novel image slicer.

    Main Results:

    • Maximal defocus between four sliced sub-images reduced from 2.363 mm to near 0.

    More Related Videos

    Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices
    07:27

    Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices

    Published on: January 31, 2019

    9.9K
    Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices
    12:04

    Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices

    Published on: May 9, 2018

    13.9K

    Related Experiment Videos

    Last Updated: Jul 31, 2025

    Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
    10:53

    Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks

    Published on: January 3, 2017

    9.9K
    Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices
    07:27

    Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices

    Published on: January 31, 2019

    9.9K
    Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices
    12:04

    Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices

    Published on: May 9, 2018

    13.9K
  • Dispersion spot diameter on the focal plane reduced from 98.47 μm to near 0.
  • Achieved a high optical transmittance of up to 91.89%.
  • Conclusions:

    • The developed non-defocus image slicer effectively eliminates image blur.
    • This technology offers significant value for high-resolution and high-transmittance spectrometer applications.