Sonication as a tool for disrupting biofilms and recovering microorganisms in bladder catheters
Juliette Cieslinski1, Victoria Stadler Tasca Ribeiro1, Camila Kowodzeichak de Lima1
1Pontifícia Universidade Católica do Paraná, Escola de Medicina, Laboratório de Doenças Infecciosas Emergentes, Curitiba, PR, Brazil.
Introduction:
Urinary catheter-related infection is commonly associated with bacterial biofilm. The impact of anaerobes is unknown, but their detection in the biofilm on this device has not been previously reported. This study aimed to evaluate the capability to recovery strict, facultative, and aerobic microorganisms in patients using bladder catheters from ICUs using conventional culture, sonication, urinary analysis, and mass spectrometry.
Methods:
Parallel, sonicated bladder catheters from 29 critically ill patients were compared with their routine urine culture. Identification was performed using matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry.
Results:
The positivity rate in urine (n = 2, 3.4%) was lower than that in sonicated catheters (n = 7, 13.8%).
Conclusion:
Bladder catheter sonication showed more positive culture results than urine samples for anaerobic and aerobic microorganisms. The role of anaerobes in urinary tract infection and catheter biofilm is discussed.
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