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Updated: Jul 31, 2025
Murine Colitis Modeling using Dextran Sulfate Sodium DSS
Published on: January 19, 2010
Intestinal Epithelial Cell-Related Alternative Splicing Events in Dextran Sodium Sulfate-Induced Acute Colitis
Background:
Alternative splicing of pre-messenger RNA is recognized as the crucial mechanism for gene expression regulation and proteome diversity generation. Alternative splicing has been found to be related to the pathogenesis of inflammatory bowel disease. The aim of this study was to identify the alternative splicing events in intestinal epithelial cells from mouse models of acute colitis and expand the understanding of the pathogenesis of inflammatory bowel disease.
Methods:
The acute colitis mouse models were constructed, and intestinal epithelial cells of the colon were isolated for RNA sequence. The replicate Multivariate Analysis of Transcript Splicing software was used to analyze the alternative splicing events. The functional analysis was performed on genes with significant differential alternative splicing events. The alternative splicing events of picked genes were validated by reverse transcription polymerase chain reaction.
Results:
A total of 340 significant differential alternative splicing events (from 293 genes) were screened out in acute colitis, and the alternative splicing events of CDK5-regulatory subunit associated protein 3 and TRM5 tRNA methyltransferase 5 were validated. The functional analysis showed that differential alternative splicing events in acute colitis participate in the apoptotic process, and the alternative splicing events of 3 genes (BCL2/adenovirus E1B-interacting protein 2, tumor necrosis factor receptor-associated factor 1, and tumor necrosis factor receptor-associated factor 7) were validated by reverse transcription polymerase chain reaction.
Conclusion:
This study pointed out the potential impact of different alternative splicing in acute colitis.
Insights
This study identified 340 alternative splicing events in mouse models of acute colitis, revealing their role in apoptosis and potential impact on inflammatory bowel disease pathogenesis.
Area of Science:
- Molecular Biology
- Genomics
- Disease Pathogenesis
Background:
- Alternative splicing of pre-messenger RNA is vital for gene expression regulation and proteome diversity.
- Alternative splicing is implicated in the pathogenesis of inflammatory bowel disease (IBD).
Purpose of the Study:
- To identify alternative splicing events in intestinal epithelial cells during acute colitis in mice.
- To enhance understanding of the molecular mechanisms underlying IBD pathogenesis.
Main Methods:
- Acute colitis mouse models were established, and colon intestinal epithelial cells were isolated for RNA sequencing.
- Multivariate Analysis of Transcript Splicing software analyzed alternative splicing events.
- Functional analysis and reverse transcription polymerase chain reaction (RT-PCR) validated key findings.
Main Results:
- 340 significant differential alternative splicing events (from 293 genes) were identified in acute colitis.
- Validated alternative splicing events in CDK5 and TRM5 genes.
- Functional analysis indicated involvement in apoptotic processes, with validation for BCL2/E1B, TRAF1, and TRAF7.
Conclusions:
- Differential alternative splicing plays a significant role in acute colitis.
- These findings highlight the potential impact of alternative splicing on IBD pathogenesis.
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