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Updated: Jul 31, 2025

Immunostaining to Visualize Murine Enteric Nervous System Development
Published on: April 29, 2015
Anatomical and functional maturation of the mid-gestation human enteric nervous system
Lori B Dershowitz1,2, Li Li3, Anca M Pasca4
1Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Abstract:
Immature gastrointestinal motility impedes preterm infant survival. The enteric nervous system controls gastrointestinal motility, yet it is unknown when the human enteric nervous system matures enough to carry out vital functions. Here we demonstrate that the second trimester human fetal enteric nervous system takes on a striped organization akin to the embryonic mouse. Further, we perform ex vivo functional assays of human fetal tissue and find that human fetal gastrointestinal motility matures in a similar progression to embryonic mouse gastrointestinal motility. Together, this provides critical knowledge, which facilitates comparisons with common animal models to advance translational disease investigations and testing of pharmacological agents to enhance gastrointestinal motility in prematurity.
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