A two-photon fluorescent probe for formaldehyde detection and regeneration in living cells
Qi Song1, Zhiqiang Liu2, Jie Niu2
1Key Laboratory of the Colloid and Interface Chemistry of the Ministry of Education, School of Chemistry and Chemical Engineering, Shandong University, Jinan, 250100, China. jiejiang@sdu.edu.cn.
Journal of Materials Chemistry. B
|May 10, 2023
Abstract:
A two-photon excited fluorescent probe CMB-1 has been rationally developed for the detection and regeneration of formaldehyde based on a novel nucleophilic addition of a secondary amine to FA and subsequential alcoholysis reactivity mechanism. It enables a specific turn-on response towards formaldehyde and facilitates the monitoring of exogenous and endogenous formaldehyde in living cells via both one- and two-photon microscopy, with minimal influence on its native homeostasis and local concentration.


