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Author Spotlight: Advancing Cellular and Protein Engineering to Control Biological Functions and Develop Novel Therapies
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Author Spotlight: Advancing Cellular and Protein Engineering to Control Biological Functions and Develop Novel Therapies

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Adenylate kinase immobilized on graphene oxide impairs progression of human lung carcinoma epithelial cells through

Anna Hetmann1, Bartosz Szymczak1, Joanna Czarnecka1

  • 1Department of Biochemistry, Faculty of Biological and Veterinary Sciences, Nicolaus Copernicus University in Torun, Torun, Poland.

Journal of Biomedical Materials Research. Part A
|May 10, 2023
PubMed

Insights

A novel nanobiocatalytic system using adenylate kinase on graphene oxide (AK-GO) effectively modulates purinergic signaling in lung cancer cells. This targeted approach reduces cancer cell proliferation and migration, offering a promising new anti-cancer therapy.

Area of Science:

  • Biochemistry
  • Nanotechnology
  • Cancer Biology

Background:

  • Purinergic signaling plays a crucial role in cancer progression.
  • Targeting purinergic signaling pathways is a promising strategy for anti-cancer therapies.
  • Adenylate kinase (AK) is involved in nucleotide metabolism within purinergic signaling.

Purpose of the Study:

  • To develop and characterize a nanobiocatalytic system (AK-GO) for modulating purinergic signaling.
  • To investigate the effects of AK-GO on ecto-nucleotide concentrations and ecto-enzyme activity in lung carcinoma cells.
  • To evaluate the anti-cancer potential of AK-GO by assessing its impact on cancer cell proliferation and migration.

Main Methods:

  • Immobilization of adenylate kinase (AK) onto graphene oxide (GO) to create the AK-GO nanobiocatalytic system.
  • Characterization of AK-GO's physicochemical and biochemical properties.
  • Treatment of human lung carcinoma epithelial (A549) cells with AK-GO.
  • Analysis of ecto-nucleotide concentrations, ecto-enzyme expression and activity, cell proliferation, and cell migration.

Main Results:

  • The AK-GO system demonstrated modified AK kinetic parameters, enabling control over ATP and adenosine concentrations.
  • AK-GO treatment led to a significant decrease in adenosinergic signaling in A549 cells.
  • The nanobiocatalytic system exhibited cyto- and hemocompatibility, indicating its suitability for biomedical applications.
  • AK-GO treatment resulted in reduced proliferation and migration capabilities of lung carcinoma cells.

Conclusions:

  • The developed AK-GO nanobiocatalytic system effectively targets and modulates purinergic signaling in lung cancer cells.
  • AK-GO shows significant potential as a novel therapeutic agent for reducing cancer cell proliferation and migration.
  • The study highlights the promise of nanobiocatalysis in developing targeted anti-cancer strategies by manipulating purinergic signaling.
Keywords:
adenosineadenylate kinaseanti-cancer activitygraphene oxideimmobilizationpurinergic signaling

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