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Complete Genome Sequences of Avian Metapneumovirus Subtype B Vaccine Strains from Brazil

Henry M Kariithi1,2, Jeremy D Volkening3, Victória Veiga Alves4

  • 1Exotic and Emerging Avian Viral Diseases Research Unit, Southeast Poultry Research Laboratory, U.S. National Poultry Research Center, USDA-ARS, Athens, Georgia, USA.

Microbiology Resource Announcements
|May 10, 2023
PubMed

Insights

Complete genome sequences of two avian metapneumovirus (aMPV) vaccine strains were determined and compared to a pathogenic field strain. This research aids in understanding aMPV-B evolution and vaccine development for poultry health.

Area of Science:

  • Veterinary Virology
  • Molecular Biology
  • Avian Pathology

Background:

  • Avian metapneumovirus (aMPV) is a significant pathogen causing respiratory and reproductive issues in poultry.
  • aMPV infections lead to substantial economic losses in the global poultry industry.

Purpose of the Study:

  • To sequence and analyze the complete genomes of two aMPV-B vaccine strains.
  • To compare these vaccine strains with a pathogenic aMPV field strain.

Main Methods:

  • Whole-genome sequencing of aMPV-B vaccine strains BR/1890/E1/19 and BR/1891/E2/19.
  • Bioinformatic analysis and comparison with the aMPV field strain VCO3/60616.

Main Results:

  • The complete genome sequences of the two aMPV-B vaccine strains were successfully obtained.
  • Comparative genomic analysis revealed genetic differences and similarities between vaccine and field strains.

Conclusions:

  • The genomic data provides a foundation for evaluating the genetic makeup of aMPV-B vaccines.
  • Understanding the genetic relationship between vaccine and field strains is crucial for effective disease control strategies.

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