Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Draft genome sequences for <i>Enterococcus mundtii</i> isolates from soil in Minnesota.

Microbiology resource announcements·2026
Same author

Characterization of a novel cell wall-associated nucleotidase of Enterococcus faecalis that degrades extracellular c-di-AMP.

PLoS pathogens·2026
Same author

Adaptation of <i>Enterococcus faecalis</i> to intestinal mucus revealed by a human colonic organoid model.

mSystems·2026
Same author

Elongation Factor Tu Acts as a Chaperone to Activate an Antibacterial RNase Toxin.

Molecular microbiology·2026
Same author

Metal uptake systems underpin <i>Enterococcus faecalis</i> virulence in both non-diabetic and diabetic wound infection models.

Infection and immunity·2025
Same author

Draft genome sequences for <i>Enterococcus mundtii</i> isolates from soil in Minnesota.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Jul 31, 2025

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
11:35

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants

Published on: May 4, 2018

8.2K

Optimized replication of arrayed bacterial mutant libraries increase access to biological resources.

Julia L E Willett1, Aaron M T Barnes1,2, Debra N Brunson3

  • 1Department of Microbiology and Immunology, University of Minnesota Medical School, Minneapolis, MN USA.

Biorxiv : the Preprint Server for Biology
|May 10, 2023
PubMed
Summary
This summary is machine-generated.

A new protocol enables the replication of large bacterial mutant libraries, ensuring genetic integrity and facilitating distribution. This method supports the creation and sharing of valuable biological resources for bacterial genetics research.

More Related Videos

Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae
12:13

Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae

Published on: April 5, 2015

10.4K
Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

46.5K

Related Experiment Videos

Last Updated: Jul 31, 2025

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants
11:35

Replication of the Ordered, Nonredundant Library of Pseudomonas aeruginosa strain PA14 Transposon Insertion Mutants

Published on: May 4, 2018

8.2K
Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae
12:13

Rapid Identification of Chemical Genetic Interactions in Saccharomyces cerevisiae

Published on: April 5, 2015

10.4K
Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays
14:06

Mapping Bacterial Functional Networks and Pathways in Escherichia Coli using Synthetic Genetic Arrays

Published on: November 12, 2012

46.5K

Area of Science:

  • Microbiology
  • Genetics

Background:

  • Arrayed mutant collections, such as transposon (Tn) or deletion mutant libraries, are crucial for accelerating bacterial genetics research.
  • Existing protocols for replicating and distributing these valuable biological resources are limited, hindering their accessibility and widespread use.
  • Standardized replication techniques are essential for maintaining the quality and utility of these collections.

Approach:

  • A detailed protocol is presented for generating multiple replicates of an arrayed bacterial Tn library comprising approximately 6,800 mutants across 73 × 96-well plates.
  • The protocol incorporates multiple quality control checkpoints to prevent contamination and minimize genetic drift during storage and handling.
  • This method is scalable, adaptable for various sizes of arrayed bacterial culture collections.

Key Points:

  • The protocol ensures high-fidelity replication of bacterial mutant libraries.
  • Quality control measures effectively prevent contamination and genetic drift.
  • The method is scalable and applicable to diverse arrayed culture collections.

Conclusions:

  • This protocol provides a robust framework for the replication and distribution of arrayed bacterial mutant libraries.
  • It serves as a valuable resource for researchers aiming to construct and share biological collections.
  • Adoption of this protocol will enhance the accessibility and utility of mutant libraries for the scientific community.