Related Experiment Video

Updated: Jul 31, 2025

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

2.7K

Solid-Phase Extraction and Enhanced Amplification-Free Detection of Pathogens Integrated by Dual-Functional

Zimu Tian, He Yan, Yong Zeng

    Medrxiv : the Preprint Server for Health Sciences
    |May 10, 2023
    PubMed
    Summary

    We developed SPEEDi-CRISPR, a novel CRISPR-Cas12a diagnostic assay. This dual-function approach enhances sensitivity for infectious disease detection without pre-amplification, offering a pragmatic solution for resource-limited settings.

    More Related Videos

    Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System07:59

    Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

    Published on: April 25, 2025

    390
    Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases10:16

    Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

    Published on: August 16, 2024

    1.3K

    Related Concept Videos

    CRISPR/Cas9 Genome Editing01:28

    CRISPR/Cas9 Genome Editing

    The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
    80
    CRISPR and crRNAs02:53

    CRISPR and crRNAs

    Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
    The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
    17.1K
    CRISPR01:59

    CRISPR

    Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
    52.5K
    The Antiviral System of Bacteria and Archaea: CRISPR01:23

    The Antiviral System of Bacteria and Archaea: CRISPR

    CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...
    63