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Related Experiment Video

Updated: Jul 31, 2025

Viability Assays for Cells in Culture
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Viability Assays for Cells in Culture

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Quantifying cell viability through organelle ratiometric probing.

Rui Chen, Kangqiang Qiu, Guanqun Han

    Biorxiv : the Preprint Server for Biology
    |May 10, 2023
    PubMed
    Summary
    This summary is machine-generated.

    Researchers developed a novel fluorescent probe, PCV-1, for single-cell viability detection using super-resolution microscopy. This probe enables dynamic visualization of cell injury and quantitative analysis of cell death mechanisms.

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    Area of Science:

    • Cell Biology
    • Biotechnology
    • Microscopy

    Background:

    • Accurate cell viability assessment is critical for precancerous cell research, treatment evaluation, and drug toxicity testing.
    • Conventional methods provide cumulative cell viability data, lacking single-cell resolution.
    • There is a need for advanced techniques to visualize and quantify cell viability at the individual cell level.

    Conclusions:

    • PCV-1 is the first probe enabling visualization of cell death and injury under super-resolution imaging.
    • The organelle ratiometric probing (ORP) assay provides a powerful tool for quantifying cell viability at the single-cell level.
    • This approach offers deeper insights into cell death mechanisms and expands the applicability of fluorescent probes in cell research.