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On the inter-monomer electron transfer in cytochrome bc1
Arkadiusz Borek1, Robert Ekiert1, Artur Osyczka1
1Department of Molecular Biophysics, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Gronostajowa 7, 30-387 Kraków, Poland.
Biochimica Et Biophysica Acta. Bioenergetics
|May 10, 2023
Summary
This study confirms electron transfer between monomers in cytochrome bc1 using mutant analysis. Further research is needed to understand the physiological role of this inter-monomer electron equilibration.
Area of Science:
- Biochemistry
- Molecular Biology
- Bioenergetics
Background:
- Cytochrome bc1 is a crucial enzyme complex functioning as a structural and functional homodimer.
- Previous studies suggested catalytically-relevant electron transfer occurs between the monomers of cytochrome bc1.
- Recent data questioning these findings necessitate further investigation into the inter-monomer electron transfer mechanism.
Purpose of the Study:
- To extend kinetic analysis of cross-dimer mutated cytochrome bc1 derivatives.
- To confirm the enzymatic origin of observed activities in these mutants.
- To explore conditions that reveal electron transfer between cytochrome bc1 monomers.
Main Methods:
- Kinetic analysis of cross-dimer mutated cytochrome bc1 derivatives.
- Enzymatic activity assays.
- Comparative analysis of mutant data to validate previous findings.
Main Results:
- Confirmed the enzymatic origin of activities in cytochrome bc1 mutants.
- Provided consistent data implicating rapid inter-monomer electron equilibration in cytochrome bc1.
- Demonstrated the relevance of these mutants in studying inter-monomer electron transfer.
Conclusions:
- The study validates the role of inter-monomer electron transfer in cytochrome bc1 function.
- Rapid electron equilibration between monomers is a consistent finding.
- The precise mechanistic and physiological significance of this inter-monomer equilibration remains to be elucidated.
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