Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Protocol for measuring cilium length using 3D confocal fluorescence microscopy, CiliaQ software, and a quality control pipeline.

STAR protocols·2025
Same author

Exploring Ciliary Mechanisms in the Causation of Hydrocephalus in Humans-Similarities and Differences from Animal Models.

Journal of molecular neuroscience : MN·2025
Same author

Advection versus diffusion in brain ventricular transport.

Fluids and barriers of the CNS·2025
Same author

Inhibition mediated by group III metabotropic glutamate receptors regulates habenula activity and defensive behaviors.

Nature communications·2025
Same author

Correction: The forkhead transcription factor Foxj1 controls vertebrate olfactory cilia biogenesis and sensory neuron differentiation.

PLoS biology·2025
Same author

Author Correction: Reliability of high-quantity human brain organoids for modeling microcephaly, glioma invasion and drug screening.

Nature communications·2025

Related Experiment Video

Updated: Jul 31, 2025

Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy
07:11

Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy

Published on: April 1, 2009

18.8K

Methods to study motile ciliated cell types in the zebrafish brain.

Percival P D'Gama1, Nathalie Jurisch-Yaksi1

  • 1Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Trondheim, Norway.

Methods in Cell Biology
|May 10, 2023
PubMed
Summary

This study presents methods for identifying motile ciliated ependymal cells in zebrafish brains. These cells are crucial for cerebrospinal fluid (CSF) movement and linked to hydrocephalus.

Keywords:
BrainCiliaEpendymal cellGlutamylated tubulinMotile ciliaMulticiliationZebrafish

More Related Videos

Visualizing the Developing Brain in Living Zebrafish using Brainbow and Time-lapse Confocal Imaging
07:28

Visualizing the Developing Brain in Living Zebrafish using Brainbow and Time-lapse Confocal Imaging

Published on: March 23, 2020

8.7K
Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos
10:52

Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos

Published on: February 3, 2010

13.5K

Related Experiment Videos

Last Updated: Jul 31, 2025

Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy
07:11

Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy

Published on: April 1, 2009

18.8K
Visualizing the Developing Brain in Living Zebrafish using Brainbow and Time-lapse Confocal Imaging
07:28

Visualizing the Developing Brain in Living Zebrafish using Brainbow and Time-lapse Confocal Imaging

Published on: March 23, 2020

8.7K
Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos
10:52

Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos

Published on: February 3, 2010

13.5K

Area of Science:

  • Neuroscience
  • Cell Biology
  • Developmental Biology

Background:

  • Cilia are conserved organelles with sensory and motile functions.
  • Motile ciliated ependymal cells line brain ventricles, driving cerebrospinal fluid (CSF) flow.
  • Ependymal cell dysfunction is implicated in hydrocephalus, a neurological disorder.

Purpose of the Study:

  • To establish and present methods for identifying and characterizing motile ciliated ependymal cells.
  • To investigate the ependymal cell lineage in the zebrafish brain.
  • To provide tools for studying ependymal cell biology and its relation to neurological conditions.

Main Methods:

  • Utilized histological staining techniques for cellular visualization.
  • Employed transgenic reporter lines for specific ependymal cell labeling.
  • Focused on the zebrafish model system for its genetic tractability.

Main Results:

  • Successfully identified and characterized motile ciliated ependymal cells in zebrafish.
  • Demonstrated the utility of histological staining and reporter lines for lineage analysis.
  • Provided a foundation for further research into ependymal cell function.

Conclusions:

  • The presented methods enable robust identification and characterization of ependymal cells.
  • Zebrafish serve as a valuable model for studying ciliated ependymal cell biology.
  • Further investigation into ependymal cells may yield insights into hydrocephalus pathogenesis.