Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Development of a gene-activated matrix for enhanced AAV gene delivery <i>in vitro</i>.

Frontiers in bioengineering and biotechnology·2026
Same author

Challenges faced by women in the diagnosis and management of polycystic ovary syndrome: a qualitative study.

Frontiers in medicine·2026
Same author

Functional asymmetry and essential structural roles of PDE6α and PDE6β subunits in rod-photoreceptor integrity.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Proximity proteomics reveals a co-evolved LRRK2-regulatory network linked to centrosomes.

EMBO reports·2026
Same author

Organelle contact reorganization drives calcium-dependent autophagy under proteostatic stress.

Autophagy·2026
Same author

Light-Activated RPE65 Inhibitors Enable On-Demand Visual Cycle Control.

Journal of the American Chemical Society·2026

Related Experiment Video

Updated: Jul 31, 2025

Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments
09:40

Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments

Published on: September 30, 2015

9.5K

Method for siRNA delivery in retina explants.

Marco Bassetto1, Merve Sen2, Florent Poulhes3

  • 1OZ Biosciences, Parc Scientifique de Luminy, CEDEX 9, Marseille, France; Gavin Herbert Eye Institute, Center for Translational Vision Research, Department of Physiology & Biophysics, University of California, Irvine, CA, United States.

Methods in Cell Biology
|May 10, 2023
PubMed
Summary
This summary is machine-generated.

Reverse Magnetofection offers a new method for non-toxic gene knockdown in adult retina explants. This technique uses magnetic nanoparticles to deliver short interfering-RNAs (siRNA) for effective RNA interference in retinal cells.

Keywords:
Magnetic nanoparticlesRNA interenceRetinal ExplantReverse Magnetofection

More Related Videos

Performing Subretinal Injections in Rodents to Deliver Retinal Pigment Epithelium Cells in Suspension
06:04

Performing Subretinal Injections in Rodents to Deliver Retinal Pigment Epithelium Cells in Suspension

Published on: January 23, 2015

17.6K
Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium
06:48

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

Published on: August 7, 2015

21.4K

Related Experiment Videos

Last Updated: Jul 31, 2025

Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments
09:40

Whole Retinal Explants from Chicken Embryos for Electroporation and Chemical Reagent Treatments

Published on: September 30, 2015

9.5K
Performing Subretinal Injections in Rodents to Deliver Retinal Pigment Epithelium Cells in Suspension
06:04

Performing Subretinal Injections in Rodents to Deliver Retinal Pigment Epithelium Cells in Suspension

Published on: January 23, 2015

17.6K
Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium
06:48

Limbal Approach-Subretinal Injection of Viral Vectors for Gene Therapy in Mice Retinal Pigment Epithelium

Published on: August 7, 2015

21.4K

Area of Science:

  • Ophthalmology and Neuroscience
  • Molecular Biology and Genetics

Background:

  • Delivering nucleic acids like RNA interference (RNAi) to the retina faces significant barriers, limiting research in retinal biology.
  • Retinal explants are valuable models, but achieving efficient and non-toxic RNAi is challenging due to cell type and structure.
  • Existing methods like electroporation and viral vectors show poor efficiency and limited cell transduction in adult retina explants.

Purpose of the Study:

  • To develop a robust and straightforward method for significant RNA interference in adult retina explants.
  • To overcome the limitations of current nucleic acid delivery techniques in retinal research.
  • To enable non-toxic gene knockdown for studying retinal biology.

Main Methods:

  • Utilized Reverse Magnetofection, a novel transfection technique.
  • Employed cationic magnetic nanoparticles (MNPs) to complex and deliver short interfering-RNAs (siRNA).
  • Applied a magnetic field to facilitate siRNA delivery into retina cells within explants.

Main Results:

  • Achieved significant RNA interference in adult retina explants.
  • Demonstrated a non-toxic method for gene knockdown.
  • Successfully delivered siRNA to retina cells using magnetic nanoparticles.

Conclusions:

  • Reverse Magnetofection provides a simple and effective tool for gene knockdown in adult retina explants.
  • This method overcomes previous limitations in achieving efficient and safe RNAi in retinal research.
  • Facilitates the study of specific gene functions in the complex environment of adult retina explants.