Related Experiment Video

Updated: Jul 30, 2025

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation
09:37

A RANKL-based Osteoclast Culture Assay of Mouse Bone Marrow to Investigate the Role of mTORC1 in Osteoclast Formation

Published on: March 15, 2018

13.3K

MiR-217 regulates autophagy through OPG/RANKL/RANK in giant cell tumors

Chenyang Meng1, Boyong Jiang1, Wanlin Liu1

  • 1The Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot, 010030, China.

Journal of Orthopaedic Surgery and Research
|May 10, 2023
PubMed
Abstract

Insights

MicroRNA-217 (miR-217) upregulation suppresses germ cell tumor (GCT) growth by inhibiting autophagy. This discovery provides a potential therapeutic target for improving GCT patient survival.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • MicroRNAs (miRNAs) are implicated in cancer development.
  • Lower miR-217 levels were observed in germ cell tumor (GCT) cells and tissues.
  • Previous studies indicated miR-217 re-expression inhibits GCT occurrence and development in vitro, but mechanisms were unclear.

Purpose of the Study:

  • To investigate the mechanisms by which miR-217 inhibits proliferation in GCT cells.
  • To explore the role of miR-217 in regulating GCT cell behavior and signaling pathways.

Main Methods:

  • Assessed GCT cell proliferation using MTT assay and BrdU staining.
  • Evaluated GCT cell migration and invasion via transwell assays.
  • Analyzed OPG/RANKL/RANK signaling pathway and autophagy-related protein expression using Western blot and RT-PCR.

Main Results:

  • miR-217 overexpression significantly suppressed GCT cell proliferation and tumorigenesis in vitro and in vivo.
  • miR-217 inhibited the OPG/RANKL/RANK signaling pathway and decreased ALP activity.
  • miR-217 suppressed autophagy-related protein expression and autophagosome/autolysosome formation in GCT cells and tissues.

Conclusions:

  • miR-217 upregulation inhibits GCT development by blocking autophagy.
  • Targeting miR-217 represents a potential therapeutic strategy for GCT patients.
  • This study offers insights into the molecular mechanisms of GCT progression and potential treatment avenues.
Keywords:
AutophagyGiant cell tumors (GCT)OPG/RANKL/RANK signal pathwaymiR-217

More Related Videos

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells09:10

siRNA Electroporation to Modulate Autophagy in Herpes Simplex Virus Type 1-Infected Monocyte-Derived Dendritic Cells

Published on: October 28, 2019

7.3K
Author Spotlight: A Selective Luciferase-Based Assay for Monitoring ATG4B 27 Activity in Cells09:51

Author Spotlight: A Selective Luciferase-Based Assay for Monitoring ATG4B 27 Activity in Cells

Published on: June 30, 2023

924

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
3.8K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Autophagy01:27

Autophagy

Autophagy is a self-digesting process by which a cell protects itself from threats both within and outside the cell, ranging from abnormal proteins to invading bacteria. In this process, obsolete components of the cell and invading microbes are degraded by hydrolytic enzymes active in an acidic environment of the lysosomal lumen.
An autophagic pathway consists of a series of signaling events activated in response to diverse stress and physiological conditions such as food deprivation,...
4.4K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.1K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
3.7K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.1K