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Perls/DAB Staining to Examine Iron Distribution in Arabidopsis Embryos
Joaquín Vargas1, Hannetz Roschzttardtz2
1Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago, Chile.
Methods in Molecular Biology (Clifton, N.J.)
|May 11, 2023
Abstract:
Iron is accumulated in Arabidopsis embryos during seed maturation. Where iron localizes in seed and embryo is important information for seed research. Iron detection can be performed in an inexpensive manner using Perls staining, based on the Prussian blue complex formation. After this first step, DAB intensification can be performed in order to visualize easily where iron pools are located in isolated embryos.
