Related Experiment Video
Updated: Jul 30, 2025
Detection of In Situ Protein-protein Complexes at the Drosophila Larval Neuromuscular Junction Using Proximity Ligation Assay
Published on: January 20, 2015
Fluorescent In Situ Detection of RNA-Protein Interactions in Intact Cells by RNA-PLA
Tianqi Li1, Wei Zhang2, Mingyi Xie3
1Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL, USA.
Abstract:
RNA-protein proximity ligation assay (RNA-PLA) enables the detection of specific RNA-protein interactions in fixed cells. In RNA-PLA, bridging and ligation of a circular DNA template occurs if the target RNA and protein are within 40 nanometers of each other. The resulting circular template is amplified by rolling circle amplification and abundantly recognized by fluorescent antisense DNA oligonucleotides. This strategy therefore enables localization of RNA-protein interactions in situ with high specificity and sensitivity. Here, we describe the use of RNA-PLA to detect interactions between a nuclear viral RNA and a host RNA-binding protein in Epstein-Barr virus (EBV)-infected B cells.
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