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Published on: April 21, 2019
Reference intervals for serum immunoglobulin A levels in Brazilian children aged 1 to 10 years: a population-based
Sandra Breder Assis1, Natasha Slhessarenko1, Cor Jesus Fernandes Fontes2
1Universidade Federal de Mato Grosso (UFMT), Faculdade de Medicina, Departamento de Pediatria, Cuiabá, MT, Brazil.
Insights
New reference intervals for serum immunoglobulin A (IgA) were established for healthy Brazilian children aged 1-11 years. These findings provide crucial data for accurate pediatric diagnosis and clinical decision-making in Brazil.
Area of Science:
- Pediatric immunology
- Clinical chemistry
- Reference interval determination
Background:
- Accurate reference intervals (RI) for serum immunoglobulin A (IgA) are essential for diagnosing immune deficiencies in children.
- Existing IgA RIs in Brazil may not accurately reflect the local pediatric population.
Purpose of the Study:
- To establish specific reference intervals (RI) for serum immunoglobulin A (IgA) levels in healthy children aged 1 to 11 years.
- To provide updated, region-specific data for Brazilian pediatricians.
Main Methods:
- A cross-sectional study involving 1,743 healthy children aged 1-11 years in Cuiabá, MT, Brazil.
- Utilized statistical methods including nonparametric bootstrap and Horn's robust method for RI determination.
- Defined RIs based on the 2.5th and 97.5th percentiles with 95% confidence intervals.
Main Results:
- Serum IgA reference intervals were determined for three age groups: 1-<2, 2-<5, and 5-<11 years.
- Sex-based partitioning was not necessary for establishing IgA RIs in this cohort.
- The derived IgA RIs differ from currently used intervals in Brazil and international studies.
Conclusions:
- Established novel serum IgA reference intervals for Brazilian children aged 1-11 years.
- These new RIs are critical for improving diagnostic accuracy in Brazilian pediatric practice.
- Facilitates informed clinical decision-making for pediatricians regarding IgA levels.
Objective:
To determine reference intervals (RI) for serum immunoglobulin A (IgA) levels in healthy children aged 1 to 1 0 years residing in the central region of Brazil.
Methods:
This cross-sectional study was conducted on 1,743 healthy children randomly selected from kindergartens and public schools in Cuiabá, MT, Brazil. The IgA RIs were defined using the statistical methods postulated by the guidelines of the United States Clinical and Laboratory Standards Institute, the nonparametric bootstrap method, and Horn's robust method after the correction of discrepancies by Tukey's, Dixon's, and Horn's methods, respectively. The results were defined based on the values contained between the 2.5th and 97.5th percentiles and their respective 95% confidence intervals.
Results:
Partition by sex was not necessary to determine the IgA RI of the studied children. Homogeneous subgroups were identified among children aged 1-<2, 2-<5, and 5-<11 years, whose IgA-specific RIs were determined.
Conclusion:
The serum IgA RIs were established for three groups of Brazilian children aged 1-11 years, which differed from those currently applied in Brazilian pediatric practice and from those defined by international studies. This definition will help Brazilian pediatricians formulate an accurate diagnosis and facilitate decision-making.
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