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Will Antigen Testing Remain Relevant in the Point-of-Care Testing Environment?

Lindsey E Nielsen¹, Steven Mahlen², Deena E Sutter³

¹LN Laboratory Consulting, NE, USA.

Clinics in Laboratory Medicine

|May 11, 2023

Summary

Traditional cell and bacterial cultures are slow and expensive for diagnosing infectious diseases. Antigen and molecular assays offer faster, more practical alternatives for timely healthcare diagnostics.

Keywords:

Antigen assay Diagnostic infectious diease assay Molecular assay Pathogen detection Point-of-care Waived testing

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Area of Science:

Clinical diagnostics
Microbiology
Infectious diseases

Background:

Cell culture was historically the gold standard for diagnosing viral and atypical infections.
Traditional culture methods are time-consuming, costly, and require specialized expertise, limiting their use in real-time healthcare settings.
Bacterial culture methods also present challenges with incubation, pathogen identification, and technical demands.

Purpose of the Study:

To discuss general considerations for implementing diagnostic assays.
To evaluate the merits of antigen and molecular assays compared to traditional methods.
To provide factors for selecting appropriate diagnostic assays for common pathogens.

Main Methods:

Review of general considerations for diagnostic assay implementation.
Discussion of antigen and molecular assay principles.
Comparative analysis of different diagnostic methodologies.

Main Results:

Antigen and molecular assays present significant advantages over traditional culture methods in terms of speed and practicality.
Implementation of new diagnostic assays requires careful consideration of various factors, including cost, expertise, and turnaround time.
These newer methods are better suited for timely diagnostic testing in diverse healthcare environments.

Conclusions:

Antigen and molecular assays are superior alternatives to traditional cell and bacterial cultures for infectious disease diagnostics.
Strategic implementation of these advanced assays is crucial for improving healthcare outcomes.
Further evaluation of assay performance and cost-effectiveness is warranted for widespread adoption.