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Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
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Label-Free Quantification of Microscopic Alignment in Engineered Tissue Scaffolds by Polarized Raman Spectroscopy.

Hui Zhou1, Janny Piñeiro Llanes2, Maedeh Lotfi1

  • 1Mechanical and Aerospace Engineering, University of Florida, Gainesville, Florida 32611, United States.

ACS Biomaterials Science & Engineering
|May 12, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed a new polarized Raman spectroscopy (PRS) method to assess microscale matrix alignment in engineered tissues. This technique, using an Amplitude Alignment Metric (AAM), offers superior accuracy over traditional methods for evaluating tissue scaffold microstructures.

Keywords:
anisotropyengineered tissue scaffoldsextracellular matrixmatrix alignmentpolarized Raman spectroscopyprincipal component analysis

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Spectroscopy

Background:

  • Monitoring extracellular matrix (ECM) microstructure is crucial for cellular processes, function, and mechanical integrity in engineered tissues.
  • Existing methods for assessing microscale alignment in engineered tissue scaffolds (ETS) have limitations.

Purpose of the Study:

  • To introduce a novel polarized Raman spectroscopy (PRS) method for quantifying microscale matrix alignment in ETS.
  • To develop a simple metric, the Amplitude Alignment Metric (AAM), for assessing matrix anisotropy.

Main Methods:

  • Derived a trained loading function from Raman spectra of native tissue using principal component analysis (PCA).
  • Applied PRS and the derived AAM to quantify alignment in various cell-derived ETS.
  • Compared PRS-based assessment with traditional microscopic methods for detecting fluorescent-labeled protein matrices.

Main Results:

  • The trained function detected prominent changes in specific Raman bands related to matrix alignment.
  • AAM significantly distinguished between anisotropic and isotropic ETS.
  • PRS method showed a lower p-value for distinguishing alignment compared to microscopic methods.

Conclusions:

  • The PRS-based method with AAM can accurately assess microscopic matrix anisotropy in complex engineered tissues.
  • This novel technique is superior to traditional microscopic methods for evaluating ETS microstructures.
  • PRS serves as a valuable tool for designing and assessing engineered tissues with native-like matrix organization.