Paenibacillus sedimenti sp. nov., isolated from freshwater wetland sediment

A Gram-stain-positive, motile, rod-shaped, facultatively anaerobic bacterium, designated strain WST5^T, isolated from sediment was characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain WST5^T was most closely related to Paenibacillus aestuarii CJ25^T (96.8 % similarity). The genome size of the WST5^T was 6.5 Mb, contained 4500 predicted protein-coding genes, and had a DNA G+C content of 46.6%. The values of whole-genome average nucleotide identity analysis and digital DNA-DNA hybridization between strain WST5^T and its closely related type strains were less than 76 and 25.6 %, respectively. The predominant cellular fatty acids (>10 %) were anteiso-C_15 : 0 and C_16 : 1 ω5c and the main menaquinone was MK-7. The major polar lipids were identified as diphospholidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and two unknown aminophospholipids. Based on the results of phenotypic, genotypic, chemotaxonomic and phylogenetic analyses, strain WST5^T is considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus sedimentum sp. nov. is proposed. The type strain is WST5^T (=NBRC 115194 ^T=CGMCC 1.18706^T).