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Updated: Jul 30, 2025
14:26
Genome-wide Purification of Extrachromosomal Circular DNA from Eukaryotic Cells
Published on: April 4, 2016
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First Insights into the Occurrence of Circular Single-Stranded DNA Genomes in Asian and African Cattle
Marie-Thérèse König1, Kai Frölich2, Anabell Jandowsky2
1Department of Veterinary Sciences, Institute of Food Science, Faculty of Veterinary Medicine, Ludwig-Maximilians-Universität München, Schönleutnerstraße 8, 85764 Oberschleißheim, Germany.
Animals : an Open Access Journal From MDPI
|May 13, 2023
Summary
Circular DNA agents, including bovine meat and milk factors (BMMF), are widespread in African and Asian cattle. These findings expand the understanding of circular DNA diversity in food-producing ruminants globally.
Area of Science:
- Virology
- Molecular Biology
- Animal Science
Background:
- Circular replicase-encoding single-stranded (CRESS) DNA viruses and other circular DNA agents are increasingly detected in various animal samples.
- Bovine meat and milk factors (BMMF) are a specific class of circular DNA agents suspected in human indirect carcinogenesis, previously linked to European cattle.
Purpose of the Study:
- To investigate the presence and diversity of BMMF and related circular DNA agents in African and Asian cattle.
- To expand the understanding of circular DNA agents in food-producing ruminants beyond European breeds.
Main Methods:
- Analysis of blood and fecal samples from African and Asian cattle.
- Detection and characterization of circular DNA molecules, including BMMF and CRESS viruses.
Main Results:
- BMMF molecules and genomoviruses were detected in all studied bovids.
- 79% of detected circular elements were BMMF groups 1 and 2; 21% were CRESS viruses (Genomoviridae family).
- Most circular DNA originated from fecal samples; novel species within Gemycircularvirus were identified.
Conclusions:
- Circular DNA agents, including BMMF, are prevalent in diverse cattle populations globally.
- This study broadens the known diversity of circular DNA in ruminants relevant to global food production.
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