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NanoLuc Luciferase as a Fluorogen-Activating Protein for GFP Chromophore Based Fluorogens
Yulia A Bogdanova1, Elvira R Zaitseva1, Alexander Yu Smirnov1,2
1Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
International Journal of Molecular Sciences
|May 13, 2023
Abstract:
In this work, we showed that the well-known NanoLuc luciferase can act as a fluorogen activating protein for various arylidene-imidazolones structurally similar to the Kaede protein chromophore. We showed that such compounds can be used as fluorescent sensors for this protein and can also be used in pairs with it in fluorescent microscopy as a genetically encoded tag.

