Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Asymmetric Lipid Bilayer01:35

Asymmetric Lipid Bilayer

7.3K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
7.3K
Formation of Lipopolysaccharides01:19

Formation of Lipopolysaccharides

57
Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin,...
57
Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

3.2K
Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
3.2K
Membrane Domains01:18

Membrane Domains

5.5K
The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the...
5.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real-world evaluation of primary prophylactic use of granulocyte colony-stimulating factor in patients with metastatic castration-sensitive prostate cancer treated with triplet therapy.

International journal of clinical oncology·2026
Same author

Activation of hamiformamide production in the thermotolerant fungus Hamigera avellanea triggered by co-culture with animal immune cells.

The Journal of antibiotics·2026
Same author

Densely Packed and Well-Aligned Liquid-Crystalline Scaffolds Drive Controllable Axial Crystal Strain.

Journal of the American Chemical Society·2026
Same author

Catenulamide A, an acylated tripeptide containing 5-methoxy-L-kynurenine lactam from an actinomycete of the genus Catenuloplanes.

The Journal of antibiotics·2026
Same author

Optimizing pelvic lymph node dissection in bladder cancer: obturator focus, pN1 prognosis, and sentinel node feasibility.

International journal of clinical oncology·2026
Same author

Safety profile of enfortumab vedotin plus pembrolizumab in locally advanced or metastatic urothelial carcinoma: a multicenter Japanese cohort study.

Japanese journal of clinical oncology·2025

Related Experiment Video

Updated: Jul 30, 2025

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
10:15

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers

Published on: July 22, 2015

14.9K

Domain Localization by Graphene Oxide in Supported Lipid Bilayers.

Ryugo Tero1, Yoshi Hagiwara1, Shun Saito1

  • 1Department of Applied Chemistry and Life Science, Toyohashi University of Technology, Toyohashi 441-8580, Japan.

International Journal of Molecular Sciences
|May 13, 2023
PubMed
Summary

Graphene oxide (GO) attracts gel-phase lipid domains in supported lipid bilayers (SLBs), influencing their distribution. This preferential localization on GO is attributed to its amphiphilic nature, guiding domain formation.

Keywords:
atomic force microscopyfluorescence microscopygraphene oxidelipid bilayer membranelipid raftphase separation

More Related Videos

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

7.0K
Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

3.6K

Related Experiment Videos

Last Updated: Jul 30, 2025

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
10:15

Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers

Published on: July 22, 2015

14.9K
Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

7.0K
Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions
12:18

Assembly of Cell Mimicking Supported and Suspended Lipid Bilayer Models for the Study of Molecular Interactions

Published on: August 3, 2021

3.6K

Area of Science:

  • Materials Science
  • Surface Chemistry
  • Biophysics

Background:

  • Supported lipid bilayers (SLBs) mimic cell membranes and are crucial for studying lipid-protein interactions.
  • Graphene oxide (GO) is a novel material with unique surface properties, including amphiphilicity.
  • Understanding lipid domain formation and localization on surfaces is key to designing advanced biomaterials.

Purpose of the Study:

  • To investigate the localization mechanism of gel-phase lipid domains on graphene oxide (GO) within supported lipid bilayers (SLBs).
  • To elucidate the role of GO's amphiphilic properties in directing lipid domain formation and distribution.
  • To explore the phase behavior of lipid bilayers containing cholesterol on GO surfaces.

Main Methods:

  • Fabrication of binary and ternary supported lipid bilayers (SLBs) on SiO2/Si substrates functionalized with graphene oxide (GO) flakes.
  • Utilizing fluorescence microscopy and atomic force microscopy (AFM) to visualize and analyze lipid domain distribution and morphology.
  • Controlled cooling rates were employed to study the effect on gel-phase domain condensation.

Main Results:

  • Gel-phase domains (composed of DPPC) preferentially localized on GO flakes, while the liquid crystalline (Lα) phase enriched the bare SiO2 surface.
  • Lower cooling rates enhanced the condensation of gel-phase domains on GO.
  • In ternary bilayers, liquid ordered (Lo) phase domains also segregated onto GO, leaving fluid components in the surrounding regions.

Conclusions:

  • Graphene oxide's amphiphilic surface properties drive the preferential nucleation and condensation of rigid lipid domains (gel and Lo phases) during SLB formation.
  • This selective localization mechanism on GO influences the overall phase distribution within the supported lipid bilayer.
  • GO serves as a platform for controlling lipid organization, with potential applications in biosensing and membrane biophysics.