Novel Strategy in Searching for Natural Compounds with Anti-Aging and Rejuvenating Potential.

Area of Science:

• Gerontology and Regenerative Medicine
• Cell Biology and Aging Research

Background:

• Defining cellular age in large populations is crucial for understanding aging processes.
• Existing methods lack quantitative approaches for assessing cell population age and rejuvenation potential.

Purpose of the Study:

• To introduce a novel cell age profile approach for identifying natural compounds with anti-aging and rejuvenation properties.
• To validate the cell age profile method using experimental data from cell cultures and in vivo studies.

Main Methods:

• Developed a cell age profile approach based on cell cycle stage distributions obtained via flow cytometry.
• Applied the method to assess the effects of a commercial substance on human mononuclear cells and dermal fibroblasts.
• Conducted in vivo testing of a selected food supplement to evaluate regenerative potential.

Main Results:

• The cell age profile method successfully identified a substance that shifted cell populations towards synthesis and proliferation stages.
• Tested substance demonstrated increased cell culture longevity and promising in vivo regenerative effects.
• The proposed methodology is generic and applicable for quantitative assessment of various anti-aging interventions.

Conclusions:

• The cell age profile approach offers a promising strategy for discovering natural compounds with significant anti-aging and rejuvenation potential.
• The methodology provides a quantitative basis for assessing regenerative interventions.
• Further research is warranted to explore molecular mechanisms and test broader applications.