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Related Concept Videos

Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

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For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
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DNA replication is carried out by a large complex of proteins that act in a coordinated matter to achieve high-fidelity DNA replication. Together this complex is known as the DNA replication machinery or the replisome.
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DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied.  After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
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The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
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The DNA Replication Fork01:02

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An organism’s genome needs to be duplicated in an efficient and error-free manner for its growth and survival. The replication fork is a Y-shaped active region where two strands of DNA are separated and replicated continuously. The coupling of DNA unzipping and complementary strand synthesis is a characteristic feature of a replication fork.   Organisms with small circular DNA, such as E. coli, often have a single origin of replication; therefore, they have only two replication...
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Related Experiment Video

Updated: Jul 30, 2025

Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
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DNA Materials Assembled from One DNA Strand.

Jiezhong Shi1, Ben Zhang1, Tianyi Zheng1

  • 1Sinopec Beijing Research Institute of Chemical Industry, Beijing 100013, China.

International Journal of Molecular Sciences
|May 13, 2023
PubMed
Summary

This review explores DNA materials made from single DNA strands, offering a simpler, cost-effective approach for applications like drug delivery and tissue engineering.

Keywords:
DNA assemblyDNA materialsnanostructureone DNA strandpalindrome

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Molecular Biology

Background:

  • DNA materials are widely used in controlled release, drug delivery, and tissue engineering due to their unique properties.
  • Current methods typically involve assembling multiple DNA strands, which can be time-consuming and prone to defects.

Purpose of the Study:

  • To review the development of DNA assembly strategies.
  • To summarize existing DNA materials constructed from a single DNA strand.
  • To discuss the principles behind single-strand DNA assembly for material creation.

Main Methods:

  • Review of existing literature on DNA assembly and single-strand DNA materials.
  • Analysis of sequence design principles, specifically palindromic sequences.
  • Discussion of factors influencing self-assembly and material formation.

Main Results:

  • Single-strand DNA assembly offers advantages in time, cost, and defect reduction compared to multi-strand methods.
  • Palindromic DNA sequences with controlled lengths are key to forming assemblies from a single strand.
  • Various DNA materials can be successfully constructed using this simplified approach.

Conclusions:

  • Single-strand DNA assembly presents a promising strategy for efficient and scalable production of DNA materials.
  • This approach has the potential to accelerate the practical application of DNA-based technologies in medicine and engineering.