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Carbocyanine-Based Optical Sensor Array for the Discrimination of Proteins and Rennet Samples Using Hypochlorite
Anna V Shik1, Irina A Stepanova1, Irina A Doroshenko1
1Department of Chemistry, M.V. Lomonosov Moscow State University, GSP-1, Leninskie Gory, 1-3, 119991 Moscow, Russia.
Sensors (Basel, Switzerland)
|May 13, 2023
Summary
This study introduces a novel method for protein discrimination using optical sensor arrays and indicator reactions. The technique rapidly identifies proteins by analyzing their oxidation products with carbocyanine dyes.
Area of Science:
- Analytical Chemistry
- Biochemistry
- Spectroscopy
Background:
- Optical sensor arrays are crucial for sample recognition and identification.
- Incorporating kinetic factors via indicator reactions enhances sensor array functionality.
- Protein analysis often requires sensitive and specific detection methods.
Purpose of the Study:
- To develop a method for discriminating proteins based on their oxidation by sodium hypochlorite.
- To utilize carbocyanine dyes for visualizing oxidation products and spectral analysis.
- To apply chemometric methods for accurate protein identification.
Main Methods:
- Proteins were oxidized by sodium hypochlorite, forming products with distinct oxidation properties.
- Carbocyanine dyes (IR-783 and Cy5.5-COOH) were used as reducing agents for visualization.
- Spectral characteristics (absorbance and fluorescence) were measured over time.
- Principal Component Analysis (PCA) and Linear Discriminant Analysis (LDA) processed image intensities.
Main Results:
- The method successfully discriminated six model proteins and ten rennet samples.
- Different spectral characteristics were registered, enabling differentiation.
- PCA and LDA effectively processed the data for recognition.
- The approach demonstrated speed and simplicity.
Conclusions:
- The proposed method offers a rapid and simple approach for protein discrimination.
- It leverages oxidation-reduction reactions and spectral analysis for identification.
- This technique is applicable to complex mixtures like rennet samples.
