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Updated: Jul 30, 2025

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Xylose Metabolization by a Saccharomyces cerevisiae Strain Isolated in Colombia
Margareth Andrea Patiño Lagos1,2, Jorge Alejandro Cristancho Caviativa3, Diana Carolina Tusso Pinzón1,2
1Facultad de Ciencias, Instituto de Biotecnología, Universidad Nacional de Colombia - Sede Bogotá, Calle 44 # 45-67 Bloque B5, oficina 703, Bogotá, Colombia.
Abstract:
Saccharomyces cerevisiae (S. cerevisiae) is the most widely used yeast in biotechnology in the world because its well-known metabolism and physiology as well as its recognized ability to ferment sugars such as hexoses. However, it does not metabolize pentoses such as arabinose and xylose, which are present in lignocellulosic biomass. Lignocellulose is a widely available raw material, with xylose content of approximately 35% of total sugars. This xylose fraction could be used to obtain high added-value chemical products such as xylitol. One of these yeasts isolated from a Colombian locality, designated as 202-3, showed interesting properties. 202-3 was identified through different approaches as a strain of S. cerevisiae, with an interesting consumption of xylose metabolizing into xylitol, in addition with excellent ability as a hexose fermenter with high ethanol yields and shows resistance to inhibitors present in lignocellulosic hydrolysates. The xylose metabolization by the 202-3 strain and their kinetics parameters had not been previously reported for any other natural strain of S. cerevisiae. These results suggest the great potential of natural strains for obtaining high value-added chemical products using sugars available in lignocellulosic biomass.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s12088-023-01054-z.
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