Mass spectrometric analysis of bioactive conditioned media of bacteria isolated from reptilian gut
Ruqaiyyah Siddiqui1,2, Noor Akbar3, Nelson Cruz Soares4,5
1College of Arts & Sciences, American University of Sharjah, University City, Sharjah, 26666, United Arab Emirates.
Aim:
To determine whether selected gut bacteria of crocodile exhibit antibacterial properties.
Materials & Methods:
Two bacteria isolated from Crocodylus porosus gut were used, namely: Pseudomonas aeruginosa and Aeromonas dhakensis. Conditioned media were tested against pathogenic bacteria and metabolites were analyzed using liquid chromatography-mass spectrometry.
Results & Conclusion:
Antibacterial assays revealed that conditioned media showed potent effects against pathogenic Gram-positive and Gram-negative bacteria. LC-MS revealed identity of 210 metabolites. The abundant metabolites were, N-Acetyl-L-tyrosine, Acetaminophen, Trans-Ferulic acid, N, N-Dimethylformamide, Pyrocatechol, Cyclohexanone, Diphenhydramine, Melatonin, Gamma-terpinene, Cysteamine, 3-phenoxypropionic acid, Indole-3-carbinol, Benzaldehyde, Benzocaine, 2-Aminobenzoic acid, 3-Methylindole. These findings suggest that crocodile gut bacteria are potential source of novel bioactive molecules that can be utilized as pre/post/antibiotics for the benefit of human health.
