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Updated: Jul 30, 2025

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

Cytocentric measurement for regenerative medicine.

Alicia D Henn¹, Taci Pereira², Joshua Hunsberger³

¹BioSpherix, Ltd. Parish, Parish, NY, United States.

Frontiers in Medical Technology

|May 14, 2023

Summary

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Regenerative Medicine (RM) requires predictable cell products. This study identifies critical cell quality attributes and technologies for monitoring cultures to ensure reliable manufacturing of cell and tissue products.

Area of Science:

Regenerative Medicine
Biotechnology
Cell Biology

Background:

Regenerative Medicine (RM) businesses need predictable cell and tissue products for regulatory approval.
Current laboratory tissue production lacks predictability and control, hindering clinical applications.
Understanding and quantifying cellular and pericellular needs is crucial for RM advancements.

Purpose of the Study:

To identify critical cell and process parameters for reproducible cell and tissue product manufacturing.
To review existing and needed technologies for monitoring cell and tissue cultures.
To emphasize the importance of cytocentric measurements for RM industry maturation.

Main Methods:

Identification of critical cell quality attributes (CCQAs).

Keywords:

Sensors cell culture techniques organoids regenerative medicine stem cells tissue engineering

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Quantification of Self-renewal in Murine Mammosphere Cultures

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Last Updated: Jul 30, 2025

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

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Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry

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Review of sensing technologies for cellular and pericellular environments.

Analysis of monitoring technologies for 2D and 3D cell cultures.

Main Results:

Key cell and process parameters influencing product quality were identified.
Available technologies for sensing these parameters were cataloged.
Gaps in current monitoring technologies for reliable manufacturing were highlighted.

Conclusions:

Cytocentric measurement of cell and tissue quality attributes is essential for the RM industry.
Standardized quality control is necessary for the maturation of RM manufacturing.
Further technological development is needed for robust monitoring of cell and tissue production.