Prediction of winter wheat leaf chlorophyll content based on VIS/NIR spectroscopy using ANN and PLSR

Visible-near-infrared spectroscopy is known for its rapid and nondestructive characteristics designed to predict leaf chlorophyll content (LCC) of winter wheat. It is believed that the nonlinear technique is preferable to the linear method. The canopy reflectance was applied to generate the LCC prediction model. To accomplish such an objective, artificial neural networks (ANN), along with partial least squares regression (PLSR), nonlinear, and linear evaluation methods have been employed and evaluated to predict wheat LCC. The wheat leaves reflectance spectra were initially preprocessed using Savitzky-Golay smoothing, differentiation (first derivative), SNV (Standard Normal Variate), MSC (Multiplicative Scatter Correction), and their combinations. Afterward, a model for LCC using the reflectance spectra was developed by means of the PLS and ANN. The vis/NIR spectroscopy samples at the 350-1400 nm wavelength were preprocessed using S. Golay smoothing, D₁, SNV, and MSC. The preprocessing with SNV-S.G, followed by PLS and ANN modeling, was able to achieve the most accurate prediction, with the correlation coefficient of 0.92 and 0.97, along with the root mean square error of 0.9131 and 0.7305 receptivity. The experimental findings also revealed that the suggested method utilizing the PLS and ANN model with SNV-S. G preprocessing was practically feasible to estimate the chlorophyll content of a particular winter wheat leaf area according to the visible and near-infrared spectroscopy sensors, achieving improved precision and accuracy. The nonlinear technique was proposed as a more refined technique for LCC estimating.